Bioz Stars score: 90/100, based on 209 PubMed citations. Immunomagnetic separation: A cell-separation technique where magnetizable microspheres or beads are first coated with monoclonal antibody, allowed to search and bind to target cells, and are then selectively removed when passed through a magnetic field.
Immunomagnetic cell separation is based on antibodies coupled to magnetic beads.

In terms of clinical usage, therapeutic cell separation allows for the introduction of enriched cell populations to a patient with a clinical need for those cells, for example, separation of leukocytes by aphaeresis or enrichment of haematopoietic stem cells by immunomagnetic separation. (1994) An immunomagnetic separation method using superparamagnetic (MACS) beads for large-scale purifi-cation of human mammary luminal and myoepithelial cells.

We combined this immunomagnetic cell separation and imaging technique with fluorescent staining, spectroscopy, and analysis to evaluate cell surface receptor-containing subpopulations, live/dead cell ratios, apoptotic/dead cell ratios, etc. Immunomagnetic cell separation is a technique whereby magnetic particles are used to isolate target cells from heterogeneous mixtures. Clarke, C., Titley, J., Davies S. C., and O’Hare, M. J. In metastasis research, it may sometimes be necessary to separate populations of tumor cells from a mixed cell population such as a tumor, peripheral blood, or bone marrow. In general, cell isolation plays a major role in a variety of biological and biomedical applications including disease management. This report describes immunomagnetic separation (IMS) techniques for enrichment and detection of viable breast carcinoma cells in BM and peripheral blood (PB).

Immunomagnetic Separation and Other Rapid Culture-Based Methods.

lectins) which specifically bind to surface antigen/receptor expressed by a given cell subset.


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(2001) Journal of Immunological Methods.256: 11-18 Figure by MIT OCW.

During incubation with a cell suspension, the antibody/bead complex binds to cells expressing the corresponding epitope.

Immunomagnetic separation (IMS) offers an alternative approach to the rapid identification of culturable and non-culturable microorganisms. Magnetic cell sorting represents an interesting evolution of immunomagnetic separation techniques. During incubation with a cell suspension, the antibody/bead complex binds to cells expressing the corresponding epitope. To accomplish this, the magnetic particles are bound to specific cell surface proteins on the target cells via antibodies, enzymes, lectins, or streptavidin. "Immunomagnetic Separation" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings).

Magnetic cell separation offers advantages of simplicity of operation, low cost and specificity and sensitivity afforded by use of immunospecific reagents. Magnetic Cell Separation: Principles and Techniques. The principles and application of the method are simple, but rely on suitable antibody specificity under the experimental conditions. A cell-separation technique where magnetizable microspheres or beads are first coated with monoclonal antibody, allowed to search and bind to target cells…


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